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Single-Cell Technologies: Advances in Single-Cell Migration and Multi-Omics
Single-cell responses to different cues are crucial for identifying the mechanisms underlying biological processes to facilitate disease detection and therapeutic development. The migration of single cells due to various stimuli is a key way in...
Emerging Biosensing Technologies for the Diagnostics of Viral Infectious Diseases
Several viral infectious diseases appear limitless since the beginning of the 21st century, expanding into pandemic lengths. Thus, there are extensive efforts to provide more efficient means of diagnosis, a better understanding of acquired immunity,...
The promise of graphene-based transistors for democratizing multiomics studies
As biological research has synthesized genomics, proteomics, metabolomics, and transcriptomics into systems biology, a new multiomics approach to biological research has emerged. Today, multiomics studies are challenging and expensive. An...
Diversification of the CRISPR Toolbox: Applications of CRISPR-Cas Systems Beyond Genome Editing
The discovery of CRISPR has revolutionized the field of genome engineering, but the potential of this technology is far from reaching its limits. In this review, we explore the broad range of applications of CRISPR technology to highlight the rapid...
Rapid and Electronic Identification and Quantification of Age-Specific Circulating Exosomes via Biologically Activated Graphene Transistors
Increasing access to modern clinical practices concomitantly extends lifespan, ironically revealing new classes of degenerative and inflammatory diseases of later years. Here, an electronic graphene field-effect transistor (gFET) is reported, termed...
Discrimination of single-point mutations in unamplified genomic DNA via Cas9 immobilized on a graphene field-effect transistor
Simple and fast methods for the detection of target genes with single-nucleotide specificity could open up genetic research and diagnostics beyond laboratory settings. We recently reported a biosensor for the electronic detection of unamplified...
Detection of Unamplified Target Genes via CRISPR–Cas9 Immobilized on a Graphene Field-effect Transistor
Most methods for the detection of nucleic acids require many reagents and expensive and bulky instrumentation. Here, we report the development and testing of a graphene-based field-effect transistor that uses clustered regularly interspaced short...
Digital Biosensing by Foundry-Fabricated Graphene Sensors
The prevailing philosophy in biological testing has been to focus on simple tests with easy to interpret information such as ELISA or lateral flow assays. At the same time, there has been a decades long understanding in device physics and...
Graphene-based biosensor for on-chip detection of bio-orthogonally labeled proteins to identify the circulating biomarkers of aging during heterochronic parabiosis
Studies of heterochronic parabiosis, where two animals of different ages are joined surgically, provided proof-of-principle results that systemic proteins have broad age-specific effects on tissue health and repair. In an effort to identify these...
Novel graphene-based biosensor for early detection of Zika virus infection
We have developed a cost-effective and portable graphene-enabled biosensor to detect Zika virus with a highly specific immobilized monoclonal antibody. Field Effect Biosensing (FEB) with monoclonal antibodies covalently linked to graphene enables...
Large scale commercial fabrication of high quality graphene-based assays for biomolecule detection
Large numbers of high quality graphene transistors with mobility approximately 5000 cm2/V*s were fabricated by chemical vapor deposition and packaged into ceramic carriers with an open cavity design. The ceramic carrier is compatible with standard...